The Preparation of Partially Purified
نویسنده
چکیده
The treatment of tuberculous meningitis is frequently hindered by the formation of exudate within the cerebrospinal spaces. This exudate may obstruct the cerebrospinal circulation and give rise to the complications of subtentorial, basal cistern, and spinal block. Furthermore, the presence of exudate predisposes to the occurrence of tuberculous arteritis, which is responsible for the cerebral and spinal cord softenings frequently found in this disease (1-4). Cathie (5) introduced the use of intrathecal streptokinase to prevent the formation of, and to remove exudate. It was hoped that the use of this agent would reduce the incidence of complications, render chemotherapy more effective, and lessen the likelihood of relapse. Clinical trials in tuberculous meningitis with streptokinase have yielded contradictory results (6-9). The majority of investigators believe that its value remains unproven. Streptokinase has no intrinsic proteolytic properties, but activates a precurser plasminogen to form a proteolytic enzyme plasmin, the action of which may be inhibited by body fluids (10). Streptokinase was used in the treatment of pleural infections by Tillett and Sherry (11), who showed that clinical response was dependent upon the demonstration of adequate fibrinolytic activity in these fluids. Investigators interested in intrathecal treatment have relied upon clinical response to assess the adequacy of the dose, and have for the most part employed a dose of 100 Christensen units (600 Cathie units) of streptokinase for intrathecal use. The development of methods for the measurement of the various components of the plasminogen-plasmin system in biological fluids (12) led Fletcher (13) to determine the composition of the cerebrospinal fluid with respect to these compo-
منابع مشابه
Physico-chemical features of Aqueous extract of acanthophyllum laxiusculum roots from natural steppe habitats of Iran: Evaluating surface activity and thermal behavior of partially purified extract
Acanthophyllum laxiusculum is one of the most widely distributed species of the genus in Iran that flourishes in steppe and mountainous regions of the country. In the present study, water-soluble content of A. laxiusculum roots was extracted by boiling water and further successively purified partially by a defined solvent system. Surface tension measurements revealed the ability of plant extrac...
متن کاملPreparation of antigens from midgut of Hyalomma anatolicum anatolicum and determining their immunoprotective effects
Partially fed (4 -5 days) H .a. anatolicum (Iranian isolate) female adult ticks were used for preparation of antigens of midgut which are, the gut supernate antigen (GS), gut membrane antigen (GM), triton extracted gut membrane antigen ( TX-GM ) and sodium dodecyl sulphate treated Triton extracted gut membrane antigen (SDS-TX-GM). The New Zealand white rabbits were immunized with GS, GM, TX- ...
متن کاملPARTIAL PURIFICATION AND CHARACTERIZATION OF B-GALACTOSIDASE FROM ASPERGILLUS NIGER UV-5
The enzyme pgalactosidase from a mutant strain of A. niger UV-5 was partially purified using ammonium sulfate and acetone. The saturation range of 60-80% ammonium sulfate was found to yield 60.5% enzyme recovery with 2.4 fold purification. Acetone precipitation at enzyme: acetone ratio of 1 : 1.5 brought about a higher yield i.e. 68% and three-fold purification. The combined procedures of ...
متن کاملEvaluation of Mango Seed Kernel Methanolic Extract on Metalloproteases in Carpet Viper (Echisocellatus) Venom: An in Vitro Experiment
Background: The global incidence of snakebite has become a major concern to the community. This study aimed to evaluate the effect of mango seed kernel methanol extract on metalloproteases in Carpet Viper (Echis ocellatus) venom. Methods: Mango seed kernel methanolic extract was evaluated in vitro for its anti-venom activity and inhibition of metalloproteases of Carpet Viper's (Echis ocellatus...
متن کاملGlycosaminoglycans (GAG) from backwater clam Marcia opima (Gmelin)
Heparin was isolated from whole clam tissue Marcia opima . Purification of multimilligram quantities of heparin from this tissue sample permitted a thorough examination of its anticoagulant activity characterization, which was partially purified by fractionation by anion exchange chromatography using DEAE cellulose column. The isolated crude and partially purified fractionated sample showed me...
متن کامل